DNA-based electrochemical biosensor using chitosan–carbon nanotubes composite film for biodetection of Pirazon
Open Access
- 1 February 2020
- journal article
- research article
- Published by Sami Publishing Company in Eurasian Chemical Communications
- Vol. 2 (2), 213-225
- https://doi.org/10.33945/sami/ecc.2020.2.7
Abstract
The interaction of Pirazon (PIR) with calf thymus ds-DNA (double-stranded Deoxyribonucleic acid) in solution and immobilized DNA on chitosan–carbon nanotubes composite modified gold electrode was investigated by electrochemical and UV-Vis spectroscopy techniques. In solution interactions, spectroscopic results indicate non-intercalative binding of PIR. A competition study with methylene blue, as an intercalative probe, was applied to confirm the binding mode of PIR. PIR showed an oxidation peak at 1.1 V at the bare Au electrode. When ds-DNA was added into the PIR solution, its peak current decreased. Au electrode modified with single wall carbon nanotube (SWCNT) and chitosan (CS) decorated with the ds-DNA were tested to determine PIR content in solution. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the electrochemical properties of the modified electrode. The modified electrode surface has good reproducibility and stability. The modified electrode exhibited linear detection range, 5×10-9 to 5×10-5 M, with a detection limit of 1×10-10 M.Keywords
This publication has 37 references indexed in Scilit:
- DNA-binding study of anthraquinone derivatives using Chemometrics methodsEuropean Journal of Medicinal Chemistry, 2011
- DNA Binding, DNA Cleavage, and Cytotoxicity Studies of Two New Copper (II) ComplexesDNA and Cell Biology, 2011
- Electrochemical studies on the binding of a carcinogenic anthraquinone dye, Purpurin (C.I. 58 205) with DNADyes and Pigments, 2010
- Voltammetric determination of trace quantities of 6-thioguanine based on the interaction with DNA at a mercury electrodeElectrochimica Acta, 2009
- Determination of Amitrole in Environmental Water Samples with Precolumn Derivatization by High-Performance Liquid ChromatographyJournal of Agricultural and Food Chemistry, 2009
- Geometrical enhancement of the proximity effect in quantum wires with extended superconducting tunnel contactsPhysical Review B, 2005
- Polymer-Anchored DNA Gene MonolayersJournal of the American Chemical Society, 2004
- Electrochemical detection of DNA hybridization using methylene blue and electro-deposited zirconia thin films on gold electrodesAnalytica Chimica Acta, 2004
- Electrochemical characterization of cetyltrimethyl ammonium bromide modified carbon paste electrode and the application in the immobilization of DNAElectrochimica Acta, 2004
- Disposable clay-coated screen-printed electrode for amitrole analysisAnalytica Chimica Acta, 2001