This study aims to determine the effectiveness of online learning using Microsoft Teams 365. The entire population in this study was used as a research sample, namely all students of the Chemistry Education study program, Indonesian Christian University, Jakarta. Perception data was collected using a questionnaire with dimensions of understanding the material, appearance and accessibility of Microsoft Teams 365. From the results of data processing, it was found that more than 50% of students' perceptions of Microsoft Teams 365 were good in all indicators of understanding the material and appearance. On the use dimension with connectivity and accessibility indicators more than 50% of students' perceptions of the Microsoft 365 team are good, but on the quota-saving indicator 42% of students do not agree. Keywords: Microsoft teams, E-learning, Cemistry