Concordance Study between the AmpFlSTR® SGM Plus™ and PowerPlex® 16 System Human Identification Kits in Bangladeshi Population

Abstract

In this study, we are reporting the concordance study between profiles from AmpFlSTR ® SGM plus™ and that of PowerPlex ® 16 system kits in Bangladeshi Bangali population. DNA samples from 234 unrelated Bangladeshi Bangali individuals were evaluated employing both the SGM plus™ and PowerPlex ® 16 system human identification kits. Complete consistency was observed in 233 out of 234 profiles which represents substantial degree of concordance amounting 99.57% between these two kits. Only one discrepancy encompassing the dropout of allele 12 at D16S539 loci in PowerPlex ® 16 system was observed. Sequence analysis revealed two mutational events; one at 16 bp distal and another next to the 3′ end of the PowerPlex 16 reverse primer of D16S539 locus. This study has shown the significance of using of alternative set of primer to address the false homozygosity issue as well as of bringing attention regarding the fact of discrepant typing from different sets of primers to the scientific community during database handling and identity or kinship investigations.