Homo Sapiens (Human) microRNA 187 Expression Is Dysregulated in Testis of Non-obstructive Azoospermic Men
Open Access
- 30 June 2020
- journal article
- Published by Maad Rayan Publishing Company in International Journal of Basic Science in Medicine
- Vol. 5 (2), 39-42
- https://doi.org/10.34172/ijbsm.2020.08
Abstract
Introduction: MiRNA expression alterations are closely related to growth, proliferation, and cell development. In addition, miR-187 has been found to regulate cellular proliferation. Further, spermatogenesis is a highly complicated process which is regulated through several genes targeted by miRNAs. However, no study has completely addressed the etiology of spermatogenic impairments. Accordingly, the present study examined the expression pattern of Homo sapiens (human) microRNA (hsa-mir-187) in the testicular biopsies of infertile men with non-obstructive azoospermia (NOA) and Sertoli cell-only syndrome (SCOS) subtype in comparison with those with obstructive azoospermia (OA). Methods: Quantitative reverse‐transcription polymerase chain reaction analysis was performed to assess the expression level of hsa-mir-187 using the ΔΔCt method. Results: Our data indicated that hsa-mir-187 was significantly up-regulated in the testis of the SCOS group (P˂ 0.05) in comparison with the OA group. Conclusion: In general, the findings suggest that the differential expression of hsa-mir-187 is linked with male infertility and shed more light on molecular defects that underlie spermatogenic impairments.Keywords
This publication has 19 references indexed in Scilit:
- MicroRNA-187, down-regulated in clear cell renal cell carcinoma and associated with lower survival, inhibits cell growth and migration though targeting B7-H3Biochemical and Biophysical Research Communications, 2013
- Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalitiesMolecular Medicine Reports, 2012
- Altered Profile of Seminal Plasma MicroRNAs in the Molecular Diagnosis of Male InfertilityClinical Chemistry, 2011
- Spermatogonial stem cell regulation and spermatogenesisPhilosophical Transactions B, 2010
- World Health Organization reference values for human semen characteristics*‡Human Reproduction Update, 2009
- Most mammalian mRNAs are conserved targets of microRNAsGenome Research, 2008
- The evolution of gene regulation by transcription factors and microRNAsNature Reviews Genetics, 2007
- In situ end-labeling of human testicular tissue demonstrates increased apoptosis in conditions of abnormal spermatogenesisFertility and Sterility, 1997
- Spontaneous germ cell death in the testis of the adult rat takes the form of apoptosis: re-evaluation of cell types that exhibit the ability to die during spermatogenesisCell Proliferation, 1996
- Bax-Deficient Mice with Lymphoid Hyperplasia and Male Germ Cell DeathScience, 1995