Protective effects of methanolic extract of Andrographis paniculata (Burm.f.) Nees leaves against arsenic-induced damage in rats

Abstract

Background: Medicinal plants are natural sources of antioxidants effective in the treatment of oxidative stress-mediated diseases. This study aims to evaluate the hepato-renal protective efficacy of Andrographis paniculata leaves methanolic extract in arsenic-induced oxidative stress. Animals were divided into four groups of six animals per group. The rats in groups 1 and 2 received normal saline, while rats in groups 3 and 4 received 200 mg/kg body weight of A. paniculata or ascorbic acid per day, respectively, for 7 days orally. The rats in groups 2, 3, and 4 received a single dose of arsenic at 10 mg/kg body weight intraperitoneally on day 7, and 24 h later, rats in all the groups were killed and the blood, liver, and kidney samples were collected for biochemical/histological studies. Results: Administration of arsenic to rats induced a significant increase in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol, urea, creatinine, and triglycerides in the plasma, while it decreased superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) activities in the liver and kidney. It also significantly reduced the levels of white blood cells (WBC), red blood cells (RBC), platelet (PLT), and lymphocytes (LYM) in the blood. However, the levels of AST, ALT, cholesterol, urea, creatinine, and triglycerides in the plasma of groups of rats that received A. paniculata extract before administration of arsenic were decreased, while their SOD, GSH, and CAT levels were elevated in the liver and kidney. The values of their WBC, RBC, PLT, and LYM were also significantly increased when compared to the arsenic group rats. Histological observations showed varying degrees of liver damage in the arsenic group rats, while the histoarchitecture of the liver of rats that received A. paniculata extract were significantly improved. Conclusions: This study demonstrated that A. paniculata extract ameliorates arsenic-induced hepato-renal toxicity and could be exploited in the management of toxicity effects associated with the arsenic.