Phosphorus supply via a fed-batch strategy improves lipid heterotrophic production of Chlorella regularis
- 4 June 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Environmental Science and Pollution Research
- Vol. 27 (25), 31677-31685
- https://doi.org/10.1007/s11356-020-09495-5
Abstract
Intracellular phosphorus (P) accumulation can improve microalgal growth and lipid synthesis. However, large excess of P causes cell poisoning. This study utilized a P-fed-batch strategy to investigate its potential to improve the utilization of the excessive P, while avoiding toxic side effects. This strategy contributed to a more complete utilization of the intracellularly stored P, which enhanced the microalgae biomass by 10−15% by upregulating the brassinosteroid growth hormone gene at a P-fed-batch frequency of 2−8. Furthermore, the lipid content increased by 4−16% via upregulation of lipid synthesis-related genes. As a result, the P-fed-batch strategy significantly increased the lipid production by 13−19%. The content of saturated fatty acid increased by ~ 100%, implying improved combustibility and oxidative stability. This is the first study of this P-fed-batch strategy and provides a new concept for the complete utilization of excessive P.Funding Information
- National Natural Science Foundation of China (51708095)
- National Natural Science Foundation of China (51578117)
- National Natural Science Foundation of China (51722803)
- Fundamental Research Funds for the Central Universities (2412017QD027, 2412018ZD013, 2412018ZD042)
- Science and Technology Project of Jilin Province (20180520168JH)
- China Postdoctoral Science Foundation (2017M611302, 2018T110241)
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