1,8-Dihydroxynaphthalene Melanin Biosynthesis in Colletotrichum fructicola Is Developmentally Regulated and Requires the Cooperative Function of Two Putative Zinc Finger Transcription Factors
- 1 October 2022
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 112 (10), 2174-2186
- https://doi.org/10.1094/phyto-01-22-0037-r
Abstract
In ascomycetes, 1,8-dihydroxynaphthalene (DHN) melanin plays important protective functions and its production is usually coupled with development and environmental stress responses. The regulation of melanin biosynthesis, however, remains obscure. Colletotrichum fructicola is a phytopathogen with a broad host range that produces melanized appressoria and perithecia. In this study, we annotated melanin genes in a high-quality C. fructicola genome and characterized two zinc finger transcription factors (TFs) (cmr1 and cmr2) that form a loosely organized gene cluster with several melanin biosynthesis genes. Deleting either TF abolished melanization in both mycelia and perithecia but did not affect appressoria. The deletion mutants also showed perithecial development defects. Overexpressing cmr1 in Δcmr2 strongly activated the expression of melanin biosynthesis genes including pks1, scd1, t4hr1, and thr1 and caused hyper-accumulation of charcoal to black pigment(s). On the other hand, overexpressing cmr2 in Δcmr1 activated pks1, t4hr1, and thr1, but not scd1. We conclude that proper DHN melanin accumulation in C. fructicola requires the cooperative function of two in-cluster TFs that also regulate perithecial development. The study clarifies DHN melanin regulations in C. fructicola and expands the function of melanin in-cluster TFs to sex regulation.Keywords
Funding Information
- National Natural Science Foundation of China (31772113)
- National Natural Science Foundation of Shaanxi (2020JQ-255)
- China Agriculture Research System of MOF and MARA (CARS27)
This publication has 47 references indexed in Scilit:
- Transcription Factor Amr1 Induces Melanin Biosynthesis and Suppresses Virulence in Alternaria brassicicolaPLoS Pathogens, 2012
- Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analysesNature Genetics, 2012
- Tri6 Is a Global Transcription Regulator in the Phytopathogen Fusarium graminearumPLoS Pathogens, 2011
- Activation of a Silent Fungal Polyketide Biosynthesis Pathway through Regulatory Cross Talk with a Cryptic Nonribosomal Peptide Synthetase Gene ClusterApplied and Environmental Microbiology, 2010
- genoPlotR: comparative gene and genome visualization in RBioinformatics, 2010
- New Biosynthetic Step in the Melanin Pathway ofWangiella(Exophiala)dermatitidis: Evidence for 2-Acetyl-1,3,6,8-Tetrahydroxynaphthalene as a Novel PrecursorEukaryotic Cell, 2008
- Analyzing real-time PCR data by the comparative CT methodNature Protocols, 2008
- Characterization of the BMR1 gene encoding a transcription factor for melanin biosynthesis genes in the phytopathogenic fungus Bipolaris oryzaeFEMS Microbiology Letters, 2008
- Melanin Biosynthesis in the Maize PathogenCochliobolus heterostrophusDepends on Two Mitogen-Activated Protein Kinases, Chk1 and Mps1, and the Transcription Factor Cmr1Eukaryotic Cell, 2007
- A Fungal Family of Transcriptional Regulators: the Zinc Cluster ProteinsMicrobiology and Molecular Biology Reviews, 2006