A case of autologous thrombofibrin clot use in a patient with post-burn persistent corneal erosion
Open Access
- 17 June 2019
- journal article
- Published by IPO Association of Transplantologists in Transplantologiya
- Vol. 11 (2), 150-157
- https://doi.org/10.23873/2074-0506-2019-11-2-150-157
Abstract
The purpose. Using clinical observation, to study the feasibility of applying a coagulant and to assess its efficacy in the treatment of patients with post-burn erosion of the cornea.Material and methods. The treatment was carried out in the Department of Trauma and Reconstructive Surgery, the Helmholtz Moscow Research Institute of Eye Diseases, Russia. Based on clinical test results, the patient, was diagnosed with post-burn persistent erosion of the cornea with sub-total cornea conjunctivization and: limbal stem cell deficiency (LSCD) in the right eye. The keratoprotective therapy conducted for several months turned ineffective. In order to activate the cornea regeneration process, the erosion site was covered with autologous thrombofibrin clot that had been obtained from the stabilized blood. Following a two-stage centrifugation of patient's stabilized blood, the plasma with the platelets were collected into a round-bottomed test-tube; and the chloride calcium activator was added. The clot was formed in the thermostat at a temperature of 37 С. The patient was given a local anesthesia, and the thrombofibrin clot was placed on the cornea surface and covered with an amniotic membrane. The membrane was fixed with a circular stitch along the limbus of the cornea.Result. The patient was followed-up and showed positive changes over time in the form of a diminished erosion area at day 5 and a complete corneal epithelialization at day 14.Summary. Our methodaimed at enhancing the reparative and regenerative processes in the cornea, reducing swelling, accelerating epithelialization, expanding transparency, and improving the optic characteristics thanks to less intensive opacity formation showed a high efficacy.This publication has 25 references indexed in Scilit:
- Autologous Serum 50% Eyedrops in the Treatment of Persistent Corneal Epithelial DefectsCornea, 2009
- Use of Autologous Platelet-Rich Plasma in the Treatment of Dormant Corneal UlcersOphthalmology, 2007
- The effect of autologous serum eyedrops in the treatment of severe dry eye disease: A prospective randomized case-control studyAmerican Journal of Ophthalmology, 2005
- The use of autologous serum tears in persistent corneal epithelial defectsEye, 2004
- Autologous serum application in the treatment of neurotrophic keratopathy*1Ophthalmology, 2004
- Comparison of autologous serum eye drops with conventional therapy in a randomised controlled crossover trial for ocular surface diseaseBritish Journal of Ophthalmology, 2004
- Autologous serum eye drops for the treatment of severe dry eye in patients with chronic graft-versus-host diseaseBone Marrow Transplantation, 2003
- Autologous serum eyedrops for dry eyes and epithelial defects: clinical and in vitro toxicity studiesBritish Journal of Ophthalmology, 2001
- Treatment of persistent corneal epithelial defect by autologous serum applicationOphthalmology, 1999
- Transforming growth factor beta-1 and beta-2 in human tear fluidCurrent Eye Research, 1996