The Effect of Apple-Derived Nanovesicles on the Osteoblastogenesis of Osteoblastic MC3T3-E1 Cells
- 1 January 2023
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Medicinal Food
- Vol. 26 (1), 49-58
- https://doi.org/10.1089/jmf.2022.k.0094
Abstract
Osteoporosis is characterized by low bone mass and elevated structural deterioration of the bone tissue, resulting in bone weakness with an increased risk of fracture. Considering biological activities of various phytochemicals extracted from apples, we herein demonstrated the potential antiosteoporotic effects of apple-derived nanovesicles (apple NVs) using osteoblastic MC3T3-E1 cells. Apple NVs significantly stimulated the growth of MC3T3-E1 cells. The cellular alkaline phosphatase (ALP) activity was significantly upregulated in the 5 μg/mL apple NVs-treated group. In addition, the concentrarion of mineralized nodules was significantly increased in the apple NVs-treated groups. Furthermore, apple NVs increased the expression of the genes and proteins associated with osteoblast growth and differentiation, such as Runx2, ALP, OPN, and BMP2/4, which further activated ERK- and JNK-related mitogen-activated protein kinase signaling. These results demonstrate that apple NVs have a potential to prevent osteoporosis by promoting osteoblastogenesis in osteoblastic MC3T3-E1 cells through regulating the BMP2/Smad1 pathways.Keywords
This publication has 46 references indexed in Scilit:
- European guidance for the diagnosis and management of osteoporosis in postmenopausal womenOsteoporosis International, 2012
- Sesamin stimulates osteoblast differentiation through p38 and ERK1/2 MAPK signaling pathwaysBMC Complementary and Alternative Medicine, 2012
- Effects of Chrysanthemum indicum L. Extract on the Function of Osteoblastic MC3T3-E1 Cells under Oxidative Stress Induced by Hydrogen PeroxideJeeKorean Journal of Food Science and Technology, 2012
- Yam Extracts Increase Cell Proliferation and Bone Matrix Protein Collagen Synthesis of Murine Osteoblastic MC3T3-E1 CellsPreventive Nutrition and Food Science, 2011
- Clinical Effectiveness of Regular Use of Unripe Apple Mask Pack on Skin Status of Middle-aged WomenJournal of Nutrition and Health, 2010
- In vitro differentiation of human mesenchymal stem cells on Ti6Al4V surfacesBiomaterials, 2008
- Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cellsGene, 2006
- Bone Morphogenetic ProteinsGrowth Factors, 2004
- Runx2 Is a Common Target of Transforming Growth Factor β1 and Bone Morphogenetic Protein 2, and Cooperation between Runx2 and Smad5 Induces Osteoblast-Specific Gene Expression in the Pluripotent Mesenchymal Precursor Cell Line C2C12Molecular and Cellular Biology, 2000
- In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.The Journal of cell biology, 1983