A comparative review of immunoassays for COVID-19 detection
Top Cited Papers
- 3 June 2021
- journal article
- review article
- Published by Taylor & Francis Ltd in Expert Review of Clinical Immunology
- Vol. 17 (6), 573-599
- https://doi.org/10.1080/1744666x.2021.1908886
Abstract
The gold standard for diagnosis of coronavirus disease 2019 (COVID-19) is detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by reverse transcription polymerase chain reaction (RT-PCR), which is expensive, time-consuming and may result in false-negative results. Serological tests can be employed for RT-PCR negative patients, contact tracing, determining the probability of protection against re-infection, and seroepidemiological studies. The main methodologies of serology-based tests for detection of SARS-CoV-2 including enzyme-linked immunosorbent assays (ELISAs), chemiluminescent immunoassays (CLIAs) and lateral flow immunoassays (LFIAs) were reviewed and their diagnostic performances were compared. Herein, a literature review on the databases of PubMed, Scopus and Google Scholar between January 01, 2020 and June 30, 2020 based on the main serological methods for COVID-19 detection with the focus on comparative experiments was performed. The review was updated on 31th December 2020. Serology testing could be considered as a part of diagnostic panel two weeks post symptom onset. Higher sensitivity for serology-based tests could be achieved by determining combined IgG/IgM titers. Furthermore, higher sensitive serological test detecting neutralization antibody could be developed by targeting spike (S) antigen. It was also demonstrated that the sensitivity of ELISA/CLIA-based methods are higher than LFIA devices.Keywords
This publication has 104 references indexed in Scilit:
- Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2Journal of Clinical Microbiology, 2020
- Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019Clinical Infectious Diseases, 2020
- Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort studyThe Lancet Infectious Diseases, 2020
- Serological Test is an Efficient Supplement of RNA Detection for Confirmation of SARS-CoV-2 InfectionPreprints.org, 2020
- A Case of 2019 Novel Coronavirus in a Pregnant Woman With Preterm DeliveryClinical Infectious Diseases, 2020
- Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2Emerging Microbes & Infections, 2020
- Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization AssayPublished by Springer Science and Business Media LLC ,2019
- Chemiluminescent immunoassay technology: what does it change in autoantibody detection?Autoimmunity Highlights, 2017
- Lateral flow assaysEssays in Biochemistry, 2016
- Assays for the assessment of neutralizing antibody activities against Severe Acute Respiratory Syndrome (SARS) associated coronavirus (SCV)Journal of Immunological Methods, 2005