miR‐377‐3p drives malignancy characteristics via upregulating GSK‐3β expression and activating NF‐κB pathway in hCRC cells
- 31 August 2017
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 119 (2), 2124-2134
- https://doi.org/10.1002/jcb.26374
Abstract
MicroRNA (miRNA) dysregulation has been associated with carcinogenesis in many cancers, including human colorectal cancer (hCRC). However, the effect and mechanism of miR‐377‐3p on CRC remains elusive. Herein, we first found that miR‐377‐3p was upregulated in CRC tissues and promoted tumorigenic activity by accelerating the G1‐S phase transition, promoting cell proliferation and epithelial‐mesenchymal transition (EMT) while repressing apoptosis in CRC cells. Glycogen synthase kinase‐3β (GSK‐3β) was a direct target of miR‐377‐3p, and upregulated by miR‐377‐3p. Knockdown of GSK‐3β partly rescued miR‐377‐3p‐mediated malignancy characteristics. Most importantly, we showed that miR‐377‐3p promoted carcinogenesis by activating NF‐κB pathway. Taken together, our results first reported that miR‐377‐3p functions as an oncogene and promotes carcinogenesis via upregulating GSK‐3β expression and activating NF‐κB pathway in hCRC cells.Keywords
Funding Information
- China Postdoctoral Science Foundation (2015M581307)
- National Natural Science Foundation of China (31270818, 81572790, 81602512, 91029714, 91629302)
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