Assessment of Antimicrobial Agents, Analgesics, and Epidermal Growth Factors-Embedded Anti-Adhesive Poly(Lactic-Co-Glycolic Acid) Nanofibrous Membranes: In vitro and in vivo Studies
Open Access
- 1 July 2021
- journal article
- research article
- Published by Taylor & Francis Ltd in International Journal of Nanomedicine
- Vol. ume 16, 4471-4480
- https://doi.org/10.2147/ijn.s318083
Abstract
Background: Postoperative tissue adhesion is a major concern for most surgeons and is a nearly unpreventable complication after abdominal or pelvic surgeries. This study explored the use of sandwich-structured antimicrobial agents, analgesics, and human epidermal growth factor (hEGF)-incorporated anti-adhesive poly(lactic-co-glycolic acid) nanofibrous membranes for surgical wounds. Materials and Methods: Electrospinning and co-axial electrospinning techniques were utilized in fabricating the membranes. After spinning, the properties of the prepared membranes were assessed. Additionally, high-performance liquid chromatography and enzyme-linked immunosorbent assays were utilized in assessing the in vitro and in vivo liberation profiles of the pharmaceuticals and the hEGF from the membranes. Results: The measured data suggest that the degradable anti-adhesive membranes discharged high levels of vancomycin/ceftazidime, ketorolac, and hEGF in vitro for more than 30, 24, and 27 days, respectively. The in vivo assessment in a rat laparotomy model indicated no adhesion in the peritoneal cavity at 14 days post-operation, demonstrating the anti-adhesive capability of the sandwich-structured nanofibrous membranes. The nanofibers also released effective levels of vancomycin, ceftazidime, and ketorolac for more than 28 days in vivo. Histological examination revealed no adverse effects. Conclusion: The outcomes of this study implied that the anti-adhesive nanofibers with sustained release of antimicrobial agents, analgesics, and growth factors might offer postoperative pain relief and infection control, as well as promote postoperative healing of surgical wounds.Keywords
This publication has 34 references indexed in Scilit:
- Postoperative Pain ControlClinics in Colon and Rectal Surgery, 2013
- Application of nanosheets as an anti-adhesion barrier in partial hepatectomyJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2013
- Function of Poly (lactic-co-glycolic acid) Nanofiber in Reduction of Adhesion BandsJournal of Surgical Research, 2012
- Pain Management After Surgery: A Brief ReviewAnesthesiology and Pain Medicine, 2012
- Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery CarrierPolymers, 2011
- Prevention of postoperative peritoneal adhesions: a review of the literatureThe American Journal of Surgery, 2011
- Application of Polycaprolactone as an Anti‐Adhesion Biomaterial FilmArtificial Organs, 2010
- The role of epidermal growth factor and its receptors in mammalian CNSCytokine & Growth Factor Reviews, 2004
- KetorolacDrugs, 1990
- CeftazidimeDrugs, 1985