Evidence for West Nile Virus and Usutu Virus Infections in Wild and Resident Birds in Germany, 2017 and 2018
Open Access
- 23 July 2019
- Vol. 11 (7), 674
- https://doi.org/10.3390/v11070674
Abstract
Wild birds play an important role as reservoir hosts and vectors for zoonotic arboviruses and foster their spread. Usutu virus (USUV) has been circulating endemically in Germany since 2011, while West Nile virus (WNV) was first diagnosed in several bird species and horses in 2018. In 2017 and 2018, we screened 1709 live wild and zoo birds with real-time polymerase chain reaction and serological assays. Moreover, organ samples from bird carcasses submitted in 2017 were investigated. Overall, 57 blood samples of the live birds (2017 and 2018), and 100 organ samples of dead birds (2017) were positive for USUV-RNA, while no WNV-RNA-positive sample was found. Phylogenetic analysis revealed the first detection of USUV lineage Europe 2 in Germany and the spread of USUV lineages Europe 3 and Africa 3 towards Northern Germany. USUV antibody prevalence rates were high in Eastern Germany in both years. On the contrary, in Northern Germany, high seroprevalence rates were first detected in 2018, with the first emergence of USUV in this region. Interestingly, high WNV-specific neutralizing antibody titers were observed in resident and short-distance migratory birds in Eastern Germany in 2018, indicating the first signs of a local WNV circulation.Keywords
This publication has 68 references indexed in Scilit:
- West Nile Virus in the United States — A Historical PerspectiveViruses, 2013
- Usutu Virus, Italy, 1996Emerging Infectious Diseases, 2013
- Pathology and tissue tropism of natural West Nile virus infection in birds: a reviewVeterinary Research, 2013
- Monitoring of West Nile Virus Infections in GermanyZoonoses and Public Health, 2012
- Epizootic Emergence of Usutu Virus in Wild and Captive Birds in GermanyPLOS ONE, 2012
- MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model SpaceSystematic Biology, 2012
- Isolation of Usutu Virus in GermanyThe American Journal of Tropical Medicine and Hygiene, 2011
- Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009Veterinary Microbiology, 2011
- Genetic Characterization of West Nile Virus Lineage 2, Greece, 2010Emerging Infectious Diseases, 2011
- Evidence of Simultaneous Circulation of West Nile and Usutu Viruses in Mosquitoes Sampled in Emilia-Romagna Region (Italy) in 2009PLOS ONE, 2010